Antisense nucleic acid technique is a method using single - stranded complementary sequences with specificity assigned by watson - crick base - pairing targeted specific messenger rna or dna to block expression of target gene 反义核酸技术是根据碱基互补原理，使用与目标靶的遗传物质( mrna或dna )特定互补的核酸片段封闭基因表达的技术方法。
Samples were taken from nonpregnant , pregnant and cultured endometrium cell , with using immunohistochemical staining , western - blot methods . to better understand this interaction , we further characterized the expression patterns of egfr messenger rnas in the mouse uterus on days 1 to 8 of pregnancy . in the present study , we found : two isofonns of non - phospho mapk was generally equally expressed in the nonpregnant and pregnant mice endometrium , and little difference among them 本研究以未孕及早孕小鼠( d1 ? d8 )的子宫内膜和培养的d4子宫内膜为样品，应用免疫组织化学、 western - blot等方法，检测了小鼠胚胎植入前后子宫内膜erk1 / erk2的表达部位及水平；用rt - pcr方法检测了egf及其受体egfr的表达情况；对培养的d4子宫内膜以le ~ y寡糖抗体阻断，观察了其对活性型mapk表达的影响。
